Development and validation of SNP genotyping assays to identify genetic sex in the swimming crab Portunus trituberculatus

The swimming crab Portunus trituberculatus is an economically vital aquaculture species in China and South East Asia. Monosex female culture has been identified as a key priority due to their increased market value, consumer preference enhanced growth performance. However, the lack of rapid reliable sex-linked marker panel hampered application sex control understanding determination species. In this study, two SNPs obtained from our simplified genomic data were validated by sequencing then converted into competitive allele-specific PCR assays. results showed high consistency between genetic phenotypic providing therefore test identification. Moreover, heterogametic genotypes males provided significant evidence support XX/XY system P. trituberculatus.